Wafaa KM Mahdi, Mohammed Sayed Mohammed and Ahmed Sameer Sanad
Background: Polycystic Ovary Syndrome (PCOS) is a common gynecological endocrine disorders; which characterized by hyperandrogenism, and anovulation with increased risks of infertility. Adiponectin is a protein specifically and abundantly expressed in adipocytes. Adiponectin Gene Polymorphisms (SNPs) influence adiponectin levels associated with risk for obesity, Insulin Resistance (IR) and T2DM. We aimed to investigate the relation between PCOS and adiponectin gene polymorphism affecting the metabolic disturbance.
Materials: Sixty women (Group 1) with PCOS were selected, had presented at gynecological-department of Minia university hospital with fertility problems. Forty healthy women (Group 2) matched with age and with regular menses and without hyperandrogenemia volunteered as controls. Serum level of F.S.H, L.H, total testosterone and glucose were measured. Adiponectin was measured by commercial immunoassays. AMH was measured by (Human AMH ELIZA kit) and insulin was measured by ELIZA, then the glucose/insulin ratio was calculated. Genomic DNA was isolated from peripheral blood leukocytes. The polymorphism was typed according to the curve obtained by real time PCR.
Results: The results showed low significant value of adiponcectin and glucose-insulin ratio was with PCOS, whereas FSH, LH, prolactin, total testosterone and AMH were higher in PCOS. Adiponectin, AMH, total testosterone, LH levels and glucose insulin ratio between PCOS and control group showed significant differences. While, there were no significant differences in case of FSH and prolactin. Plasma adiponectin concentration was positively correlated with insulin-stimulated glucose disposal with a significant of 0.003. The genotyping distributions of TG, GG and TT in women with PCOS are 22 (37%), 19 (31.5%) and 19 (31.5%), respectively. The correlation of adiponcetin and genotyping is significant P = 0.001.
Conclusion: Prevalence of adiponectin gene polymorphism is higher in PCOS, with significant correlation with the glucose/insulin ratio.
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