Blanca Chavez Sandoval*
The integration of nanotechnology, with molecular biology and electrochemistry, have created a great expectation due to the development of new nucleic acid detection strategies based on nanomaterials and which are important tools in genomics, medical diagnosis, pharmacogenetics, pathology, criminology, food security, environmental monitoring, among others; particularly in this stage of the COVID-19 pandemic.
In this work, the DNA detection of four microorganisms was performed: Escherichia coli; Aspergillus niger; Salmonella typhi and Achlya sp by electrochemical detection at the DNA level in water, using nanostructured genosensors, which represent a simple device; quick; accurate and economical for the detection of microorganisms.
The specific probes were designed with the primer Blast program, they were synthesized and used with Gold Nanoparticles (AuNPs) as a brand; the probes were functionalized with the AuNPs by the streptavidin-biotin system and hybridization was performed, which was detected electrochemically, and it was observed that the signal decreases when there is less complementarity between the probe and the analytic, with the aim of the developed genosensors to be a tool for the rapid detection of microorganisms for purposes of monitoring, prevention and environmental control among others, like COVID-19; furthermore, if genosensors are produced on a large scale, they can be efficient, inexpensive, portable and simple to use.
Keywords Genosensors; Microorganisms; DNA probes; Nanoparticles
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