Olawale Onada
The freshwater drum, or Aplodinotus grunniens, is a common type of eurythermal freshwater fish in North America. New aquaculture opportunities emerged as a result of our team's significant advancements in artificial breeding and cultivation in 2019 and research into the physiological responses of the organisms to their surroundings. Its capacity to adapt to hypothermia and maintain metabolic homeostasis, on the other hand, is poorly understood. This experiment used cold stress for eight days at temperatures of 18 °C (LT18) and 10 °C (LT10), with a control temperature of 25 °C (Con), in order to investigate the effects of shortterm hypothermia on the physiology and metabolism of freshwater drum. LT18 and LT10 had significantly lower levels of free essential amino acids than Con after two days of cold stress. At LT10, both the activity of lipase (LPS) and the amount of total triglyceride (TG) in the plasma decreased over the course of two days. According to RNA-seq in the liver, metabolic-related signaling, particularly amino acid synthesis and lipid metabolism, was prevented by hypothermia. The PPAR signaling pathway is specifically connected to hypothermia-induced inhibition of lipid and amino acid metabolism. These findings confirmed that PPAR signaling maintains lipid and amino acid metabolic homeostasis under cold stress. These results theoretically support hypothermia resistance in the metabolic homeostasis of freshwater drums.
KeywordsHypothermia; Lipid Metabolism; Amino Acid Metabolism; Ppar Signaling
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