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Abstrato

Single strand conformation polymorphism: Principle and Troubleshoots

Mona Sharma

One of the common techniques used to identify unknown mutations is single strand conformation polymorphism (SSCP).SSCP is used frequently because the technique is simple, fast, sensitive and reproducible. It has been frequently reported that it is very easy to establish this technique with minimal infrastructure and expenditure. This short communication presents the basic principles of SSCP and possible troubleshoots of standardization of this technique in the lab.